In silico chromosome staining

In silico chromosome staining: reconstruction of Giemsa bands from the whole human genome sequence.

Giemsa staining has been used for identifying individual human chromosomes. Giemsa-dark and -light bands generally are thought to correspond to GC-poor and GC-rich regions; however, several experiments showed that the correspondence is quite poor. To elucidate the precise relationship between GC content and Giemsa banding patterns, we developed an "in silico chromosome staining" method for reco...

Staining Techniques for Human Chromosome Identification

An accepted feature of technological development and its associated accumulation of data is that progress occurs in an irregular fashion with 'break throughs' being interspersed by periods of relative inactivity. In the halcyon years of 1955 to 1961, human cytogenetics experienced a revolution which brought the field within the scope of most genetic laboratories. The resulting increase in the v...

Chromosome variation within Drosophila simulans detected by quinacrine staining.

HEN stained with quinacrine o r nitrogen mustard derivatives of quinacrine, certain regions of Drosophila salivary gland chromosomes fluoresce with extraordinary brilliance (VOSA 1970; ELLISON and BARR 1971). Although the biochemical specificity of this reaction is not well understood, the method of quinacrine staining can be used for cytological comparisons of chromosomes among species and wit...

an acetocarmine staining procedure for chromosome banding studies of immature pollen in triticeae

studies of postmeiotic chromosomes have been impeded by the thick exine of immature pollen grains. chromosome staining of immature and adult pollen grains through conven-tional acetocarmine procedure is tedious and often leads to unsatisfactory results. an acetocarmine stain, used in conjugation with carnoy’s solution as fixative for 72 hrs or until the chlorophyll-containing tissues of spike b...

Short communication: Chromosome description and localization of Nucleolus Organizing Regions (NORs) by Ag-staining Technique in Alburnus filippii (Cyprinidae, Cypriniformes) in Anzali Lagoon, North of Iran